Application
MMP8 antibody immunoprecipitates MMP8 protein in IP experiments. IP samples: A549 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of MMP8 protein by 4 μg MMP8 antibody 10 % SDS-PAGE The immunoprecipitated MMP8 protein was detected by MMP8 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
Immunofluorescence analysis of methanol-fixed A431, using MMP8 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using MMP8 antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: HCT116 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | MMP8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MMP8 (neutrophil collagenase)Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage. Its function is degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MMP8. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CLG1 antibody HNC antibody MMP-8 antibody PMNL-CL antibody MMP8 antibody neutrophil collagenase antibody PMNL collagenase antibody matrix metalloproteinase-8 antibody matrix metalloproteinase 8 (neutrophil collagenase) antibody matrix metallopepti |
|---|---|
| Molecular Weight(MW) | 53 kDa (note) (3) |
| Cellular Localization | Cytoplasmic granule , Secreted , extracellular space , extracellular matrix (Probable) |
Application
MMP8 antibody immunoprecipitates MMP8 protein in IP experiments. IP samples: A549 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of MMP8 protein by 4 μg MMP8 antibody 10 % SDS-PAGE The immunoprecipitated MMP8 protein was detected by MMP8 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
Immunofluorescence analysis of methanol-fixed A431, using MMP8 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using MMP8 antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: HCT116 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
