Application
ELISA analysis of control antigen and antigen using MPL antibody [1H2].Application
FACS analysis of MOLT4 cells using MPL antibody [1H2] (green) or a negative control (red).Application
WB analysis of human MPL (a.a. 307-362) recombinant protein using MPL antibody [1H2].| Product Name | MPL antibody [1H2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [1H2] to MPLIn 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 |
| Immunogen | The immunogen corresponding to human MPL expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 1H2 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSWB |
| ELISA: 1/10000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | MPLV , TPOR , CD110 , THCYT2 , C-MPL |
|---|---|
| Target | MPL |
| Molecular Weight(MW) | 71.2 kDa (note) |
Application
ELISA analysis of control antigen and antigen using MPL antibody [1H2].
Application
FACS analysis of MOLT4 cells using MPL antibody [1H2] (green) or a negative control (red).
Application
WB analysis of human MPL (a.a. 307-362) recombinant protein using MPL antibody [1H2].| Application Notes | ELISA: 1/10000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer. |
