| Product Name | MPS1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [N1] to MPS1TTK protein kinase (MPS 1) is essential for establishment of spindle assembly checkpoint, but dispensable for centrosome duplication. |
| Immunogen | Recombinant near full length protein (catalytically inactive - D663A), corresponding to amino acids 3-856 of Human MPS1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | N1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-FIPWB |
| ELISA: Use at an assay dependent dilution. FuncS: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000. Detects a band of approximately 97 kDa. Optimal dilutions/concentrations should be determined by the end user. This antibody has been used for microinjection experiments, where it has been used to interfere with the spindle assembly checkpoint (Stucke et al. 2002). For IF, it is recommended that cells are fixed in 4% paraformaldehyde in PBS and permeabilized with 0.2% Triton X-100. Methanol and acetone fixatives can also be used. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | MPS-1, MPS1, 6232, 603702, P42677, RPS27, MPS 1 |
|---|---|
| Target | MPS1 |
| Tissue Specificity | This antibody was shown to specifically detect MPS1 in Stucke et al. 2002. |
| Application Notes | ELISA: Use at an assay dependent dilution. FuncS: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000. Detects a band of approximately 97 kDa. Optimal dilutions/concentrations should be determined by the end user. This antibody has been used for microinjection experiments, where it has been used to interfere with the spindle assembly checkpoint (Stucke et al. 2002). For IF, it is recommended that cells are fixed in 4% paraformaldehyde in PBS and permeabilized with 0.2% Triton X-100. Methanol and acetone fixatives can also be used. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS |
