Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] diluted at 1:1000.Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Application
Mre11 antibody [12D7] detects Mre11 protein by western blot analysis. A. 30 µg Jurkat whole cell extract B. 30 µg Raji whole cell extract C. 30 µg NCI-H929 whole cell extract 7.5% SDS-PAGE Mre11 antibody [12D7] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Mre11 antibody [12D7] detects Mre11 protein by western blot analysis. A. 30 µg 293T whole cell extract B. 30 µg whole cell extract of human Mre11-transfected 293T cells 7.5% SDS-PAGE Mre11 antibody [12D7] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Analysis of human MRE11 expression by western blot in HeLa (1) and Raji (2) whole cell extracts using anti-MRE11 [12D7] monoclonal antibody . The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Product Name | Mre11 antibody [12D7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [12D7] to MRE11Mre11 is an 80 kDa protein that acts as a Mn+2 dependent double stranded DNA 3' to 5' exonuclease and a single stranded DNA endonuclease. It is part of a larger complex with Rad50, p95 (Nibrin) and possibly other associated proteins involved in the nucleolytic end-processing of double strand breaks and telomere maintenance. |
| Immunogen | Amino acids 182-582 of Mre11 expressed in E. coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 12D7 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHC-PIPWBFunctional Assay |
| For WB: Use at a dilution of 1:500-1:3000. For ICC/IF: Use at a dilution of 1:100 - 1:200. For IHC-P: Use at a dilution of 1:200. Incubation with the primary antibody was preceded by microwave treatment of the tissue section for antigen retrieval. For IP: Use at a dilution of 1:100. Predicted molecular weight: 79 kDa. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MRE11, MRE11B, P49959, 600814, ATLD, 4361, MRE11A, HNGS1 |
|---|---|
| Target | Mre11 |
| Molecular Weight(MW) | 80 kDa (note) |
| Cellular Localization | Nuclear |
| Light Chain Type | kappa |
Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] diluted at 1:1000.
Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Application
Mre11 antibody [12D7] detects Mre11 protein by western blot analysis. A. 30 µg Jurkat whole cell extract B. 30 µg Raji whole cell extract C. 30 µg NCI-H929 whole cell extract 7.5% SDS-PAGE Mre11 antibody [12D7] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Mre11 antibody [12D7] detects Mre11 protein by western blot analysis. A. 30 µg 293T whole cell extract B. 30 µg whole cell extract of human Mre11-transfected 293T cells 7.5% SDS-PAGE Mre11 antibody [12D7] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Analysis of human MRE11 expression by western blot in HeLa (1) and Raji (2) whole cell extracts using anti-MRE11 [12D7] monoclonal antibody . The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Positive Control | HeLa , Jurkat , Raji , NCI-H929 , 293T , human Mre11-transfected 293T |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:500-1:3000. For ICC/IF: Use at a dilution of 1:100 - 1:200. For IHC-P: Use at a dilution of 1:200. Incubation with the primary antibody was preceded by microwave treatment of the tissue section for antigen retrieval. For IP: Use at a dilution of 1:100. Predicted molecular weight: 79 kDa. Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing no preservatives |
