Mouse monoclonal [M2I - 4] to MRP2Canalicular Multi Organic Anion Transporter / Multidrug Resistance Associated Protein 2 (cMOAT / MRP 2) is a membrane transport protein with a molecular weight of 170-180 kD.
Immunogen
Bacterial fusion protein of human MRP 2 containing amino acids of 215-310.
Key Feature
Clonality
Monoclonal
Isotype
IgG
Clone Number
M2I-4
Host Species
Mouse
Tested Applications
FACSICC/IFIHC-FWB
ICC/IF:Acetone fixed cytospin preparations:Use at a 1:20-1:50 dilution.IHC:Acetone-fixed frozen sections of human tissues:Use at a 1:20 dilution.Follow by incubating with rabbit anti-mouse IgG and a monoclonal mouse APAAP complex.Alternatively,slides can be incubated with biotinylated rabbit anti-mouse IgG and streptavidin conjugated to horseradish peroxidase.
WB:Use at a 1:20-1:50 dilution and anti-mouse HRP.
Optimal dilutions / concentrations should be determined by the end user.
Found on the apical membrane of polarized cells in liver, kidney and intestine.
Application Notes
ICC/IF:Acetone fixed cytospin preparations:Use at a 1:20-1:50 dilution.IHC:Acetone-fixed frozen sections of human tissues:Use at a 1:20 dilution.Follow by incubating with rabbit anti-mouse IgG and a monoclonal mouse APAAP complex.Alternatively,slides can be incubated with biotinylated rabbit anti-mouse IgG and streptavidin conjugated to horseradish peroxidase.
WB:Use at a 1:20-1:50 dilution and anti-mouse HRP.
Optimal dilutions / concentrations should be determined by the end user.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution, aliquot and store at 4°C.