| Product Name | MRP3 antibody [M3II-21] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [M3II - 21] to MRP3Human multidrug-resistance protein (MRP) gene family is composed of multiple members including MRP 1, MRP 2 or cMOAT and four other homologous genes called MRP 3, MRP 4, MRP 5 and MRP 6. Canalicular Multi Organic Anion Transporter 2 / Multidrug Resistance Associated Protein 3 (cMOAT 2 / MRP 3) has a molecular weight of 190-200 kD. MRP3 is closest to MRP1 with 58% amino acid identity. MRP 3 has been shown to be mainly expressed in the liver, colon, intestine, adrenal gland and, to a lower extent, in several other tissues. |
| Immunogen | Bacterial fusion protein of human MRP 3 containing amino acids 830-949. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | M3II-21 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHC-FWB |
| Immunocytochemistry: use 1:20-1:50 dilution on acetone or formaldehyde fixed cytospin preparations. Immunohistochemistry: use 1:20 on acetone fixed frozen sections. Western blotting: use 1:20-1:50 dilution and anti mouse HRP. Flow cytometry: optimal conditions still to be defined. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Synonyms | cMOAT2, O15438, MRP3, MLP2, ABC31, 604323, MOAT-D, EST90757, 8714, MOATD, ABCC3, MOAT D |
|---|---|
| Target | MRP3 |
| Tissue Specificity | M3II-21 reacts with an internal epitope of MRP3, a 190-200 kD transmembrane protein that is closely related to the multidrug resistance protein MRP1. M3II-21 was raised against a bacterial fusion protein of MRP3, containing amino acids 830-949 of the protein. M3II-21 does not cross-react with the human MDR1, MRP1, MRP2 or MRP5 gene products. |
| Cellular Localization | Cell Membrane |
| Application Notes | Immunocytochemistry: use 1:20-1:50 dilution on acetone or formaldehyde fixed cytospin preparations. Immunohistochemistry: use 1:20 on acetone fixed frozen sections. Western blotting: use 1:20-1:50 dilution and anti mouse HRP. Flow cytometry: optimal conditions still to be defined. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C for short term (3 months) and at -20°C for extended storage. |
| Storage Buffer | 0.7% BSA and 0.1% sodium azide. |
