Application
WB analysis of (1) HL7702, (2) HepG2, (3) SMMC-7721, (4) HEK293, (5) HeLa and (6) Raji lysates using MRPL42 antibody [3H6G11].Application
ELISA analysis of control antigen and antigen using MRPL42 antibody [3H6G11].Application
FACS analysis of HepG2 cells using MRPL42 antibody [3H6G11] (green) or a negative control (purple).Application
ICC/IF analysis of HeLa cells using MRPL42 antibody [3H6G11] (green) and DRAQ5 fluorescent DNA dye (blue).Application
IHC-P analysis of cervical cancer tissues using MRPL42 antibody [3H6G11].Application
IHC-P analysis of esophageal cancer tissues using MRPL42 antibody [3H6G11].Application
WB analysis of lysates from (1) untransfected and (2) MRPL42 (a.a. 142-203)-hIgGFc transfected HEK293 cells using MRPL42 antibody [3H6G11].Application
WB analysis of human MRPL42 recombinant protein using MRPL42 antibody [3H6G11].| Product Name | MRPL42 antibody [3H6G11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [3H6G11] to MRPL42Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q. [provided by RefSeq, May 2011] |
| Immunogen | The immunogen corresponding to a region within amino acids 142 and 203 of human MRPL42 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3H6G11 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| ELISA: 1/10000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | PTD007 , HSPC204 , L31MT , MRP-L42 , L42MT , MRP-L31 , S32MT , MRPL31 , MRPS32 , MRP-S32 , RPML31 |
|---|---|
| Target | MRPL42 |
| Molecular Weight(MW) | 16.7 kDa (note) |
Application
WB analysis of (1) HL7702, (2) HepG2, (3) SMMC-7721, (4) HEK293, (5) HeLa and (6) Raji lysates using MRPL42 antibody [3H6G11].
Application
ELISA analysis of control antigen and antigen using MRPL42 antibody [3H6G11].
Application
FACS analysis of HepG2 cells using MRPL42 antibody [3H6G11] (green) or a negative control (purple).
Application
ICC/IF analysis of HeLa cells using MRPL42 antibody [3H6G11] (green) and DRAQ5 fluorescent DNA dye (blue).
Application
IHC-P analysis of cervical cancer tissues using MRPL42 antibody [3H6G11].
Application
IHC-P analysis of esophageal cancer tissues using MRPL42 antibody [3H6G11].
Application
WB analysis of lysates from (1) untransfected and (2) MRPL42 (a.a. 142-203)-hIgGFc transfected HEK293 cells using MRPL42 antibody [3H6G11].
Application
WB analysis of human MRPL42 recombinant protein using MRPL42 antibody [3H6G11].| Application Notes | ELISA: 1/10000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer. |
