Application
ELISA analysis of control antigen and antigen using MSH6 antibody [3E1].Application
FACS analysis of MCF-7 cells using MSH6 antibody [3E1] (green) or a negative control (red).Application
ICC/IF analysis of HeLa cells using MSH6 antibody [3E1] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).Application
IHC-P analysis of colon cancer tissues using MSH6 antibody [3E1].Application
IHC-P analysis of rectum cancer tissues using MSH6 antibody [3E1].Application
WB analysis of lysates from (1) HEK293, (2) HCT116, (3) A549, (4) A431, (5) MCF-7 and (6) HepG2 lysates using MSH6 antibody [3E1].Application
WB analysis of human MSH6 (a.a. 217-395) recombinant protein using MSH6 antibody [3E1].| Product Name | MSH6 antibody [3E1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [3E1] to MSH6This gene encodes a protein similar to the MutS protein. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides, prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein of this gene combines with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene have been identified in individuals with hereditary nonpolyposis colon cancer (HNPCC) and endometrial cancer. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to human MSH6 expressed in E. Coli.;. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 3E1 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| ELISA: 1/10000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HNPCC5 , GTBP , HSAP |
|---|---|
| Target | MSH6 |
| Molecular Weight(MW) | 160 kDa (note) |
Application
ELISA analysis of control antigen and antigen using MSH6 antibody [3E1].
Application
FACS analysis of MCF-7 cells using MSH6 antibody [3E1] (green) or a negative control (red).
Application
ICC/IF analysis of HeLa cells using MSH6 antibody [3E1] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).
Application
IHC-P analysis of colon cancer tissues using MSH6 antibody [3E1].
Application
IHC-P analysis of rectum cancer tissues using MSH6 antibody [3E1].
Application
WB analysis of lysates from (1) HEK293, (2) HCT116, (3) A549, (4) A431, (5) MCF-7 and (6) HepG2 lysates using MSH6 antibody [3E1].
Application
WB analysis of human MSH6 (a.a. 217-395) recombinant protein using MSH6 antibody [3E1].| Application Notes | ELISA: 1/10000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
