Application
Formalin fixed paraffin embedded human colon carcinoma stained with MSH6 antibody (cat. ).Application
IHC Image using - Detection of MSH6 by IHC in Human Colon Carcinoma| Product Name | MSH6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [44] to MSH6MSH6 is a heterodimer of MSH2 and binds to DNA containing G/T mismatches. The MSH2-MSH6 complex recognizes a single-based mispair insertion/deletion loop. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related tuman carcinogenesis. Studies have shown the mutations of MLH-1, MSH2 and MSH6 genes contribute to the development of sporadic colorectal carcinoma. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. |
| Immunogen | Synthetic peptide human MSH6 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 44 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-PWB |
| For WB:Use at 1:100-1:500. For IHC-P: Use at 1:25-1:50 in an ABC method for 30-60 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 600678 GTBP HSAP P52701 MSH6 HNPCC5 2956 |
|---|---|
| Target | MSH6 |
| Cellular Localization | Nuclear |
Application
Formalin fixed paraffin embedded human colon carcinoma stained with MSH6 antibody (cat. ).
Application
IHC Image using - Detection of MSH6 by IHC in Human Colon Carcinoma| Positive Control | Colon carcinoma & Tonsil |
|---|---|
| Application Notes | For WB:Use at 1:100-1:500. For IHC-P: Use at 1:25-1:50 in an ABC method for 30-60 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
