Application
WB analysis of SW480 lysate (10 μg) using MSH6 antibody [EPR3945] at a dilution of 1:6000.Application
WB analysis of rat brain lysate (10 μg) using MSH6 antibody [EPR3945] at a dilution of 1:1000.| Product Name | MSH6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EPR3945] to MSH6This gene encodes a protein similar to the MutS protein. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides, prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein of this gene combines with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene have been identified in individuals with hereditary nonpolyposis colon cancer (HNPCC) and endometrial cancer. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic peptide corresponding to residues at the N terminus of Human MSH6. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EPR3945 |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| IHC-P: 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. WB: 1/1000 - 1/10000. ICC/IF: 1/250.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | mutS homolog 6 (E. coli) HNPCC5 HSAP GTBP |
|---|---|
| Target | MSH6 |
| Molecular Weight(MW) | 163 kDa (note) |
Application
WB analysis of SW480 lysate (10 μg) using MSH6 antibody [EPR3945] at a dilution of 1:6000.
Application
WB analysis of rat brain lysate (10 μg) using MSH6 antibody [EPR3945] at a dilution of 1:1000.| Application Notes | IHC-P: 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. WB: 1/1000 - 1/10000. ICC/IF: 1/250.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for 12 months at -20°C |
| Storage Buffer | PBS containing 50% Glycerol, 0.05% BSA and 0.01% Sodium azide. |
