Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTMR14 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTMR14.Application
HEK293T cells transfected with either RC207732 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MTMR14 antibody, and then analyzed by flow cytometry.Application
Anti-MTMR14 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with MTMR14| Product Name | MTMR14 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [7F2] to MTMR14Lipid phosphatase which efficiently dephosphorylates phosphatidylinositol 3-phosphate (PtdIns3P) and PtdIns(3,5)P2 | inactive toward PtdIns4P, PtdIns(3,4)P2, PtdIns(4,5)P2 and PtdIns(3,4,5)P3. |
| Immunogen | Full length human recombinant protein of human MTMR14 (NP_001070993) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 7F2 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| ICC/IF 1:100 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ22405 MTMR14 FLJ90311 |
|---|---|
| Target | MTMR14 |
| Tissue Specificity | is specific for human MTMR14 (NP_001070993). |
| Cellular Localization | Found in reticular structures and plasma membrane ruffles. Concentrated near the nucleus. |
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTMR14 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTMR14.
Application
HEK293T cells transfected with either RC207732 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MTMR14 antibody, and then analyzed by flow cytometry.
Application
Anti-MTMR14 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with MTMR14| Application Notes | ICC/IF 1:100 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
