Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C1], Internal diluted at 1:500.Application
mTOR antibody [C1], Internal detects mTOR protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C1], Internal diluted at 1:500.Application
mTOR antibody [C1], Internal detects mTOR protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C1], Internal diluted at 1:1000.Application
mTOR antibody detects mTOR protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody diluted at a dilution of 1:1000.| Product Name | mTOR antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to mTOR (FK506 binding protein 12-rapamycin associated protein 1)The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human mTOR. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, SheepZebrafish (94%), Sheep (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ44809 antibody FRAP antibody FRAP1 antibody FRAP2 antibody RAFT1 antibody RAPT1 antibody MTOR antibody FK506 binding protein 12-rapamycin associated protein 2 antibody FK506-binding protein 12-rapamycin complex-associated protein 1 antibody FKB |
|---|---|
| Target | mTOR |
| Molecular Weight(MW) | 289 kDa (note) |
| Cellular Localization | Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side , Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side , Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C1], Internal diluted at 1:500.
Application
mTOR antibody [C1], Internal detects mTOR protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C1], Internal diluted at 1:500.
Application
mTOR antibody [C1], Internal detects mTOR protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C1], Internal diluted at 1:1000.
Application
mTOR antibody detects mTOR protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody diluted at a dilution of 1:1000.| Positive Control | HeLa , Jurkat , Raji , NCI-H929 , Rat brain , Mouse brain , 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
