Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C3], C-term diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
mTOR antibody [C3], C-term detects mTOR protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. mTOR antibody [C3], C-term diluted at 1:500.Application
mTOR antibody detects mTOR protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 100% MeOH for 5 min. Green: mTOR protein stained by mTOR antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Cross-linked ChIP was performed with 293T chromatin extract and 5 μg of either control rabbit IgG or anti-mTOR antibody. The precipitated DNA was detected by PCR with primer set targeting to 45S rDNA promoter .Application
Immunoprecipitation of mTOR protein from 293T whole cell extracts using 5 μg of mTOR antibody [C3], C-term . Western blot analysis was performed using mTOR antibody [C3], C-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
mTOR antibody immunoprecipitates mTOR protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of mTOR by 3 μg of mTOR antibody 5% SDS-PAGE The immunoprecipitated mTOR protein was detected by mTOR antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.Application
mTOR antibody detects FRAP1 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. mTOR antibody dilution: 1:500.Application
mTOR antibody detects mTOR protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
mTOR antibody detects mTOR protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
mTOR antibody [C3], C-term detects mTOR protein at mitochondria on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. mTOR antibody [C3], C-term diluted at 1:500.| Product Name | mTOR antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to mTOR (mechanistic target of rapamycin (serine/threonine kinase))The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human mTOR. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, SheepZebrafish (90%), Sheep (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ44809 antibody FRAP antibody FRAP1 antibody FRAP2 antibody RAFT1 antibody RAPT1 antibody MTOR antibody FK506 binding protein 12-rapamycin associated protein 2 antibody FK506-binding protein 12-rapamycin complex-associated protein 1 antibody FKB |
|---|---|
| Molecular Weight(MW) | 289 kDa (note) |
| Cellular Localization | Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side , Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side , Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C3], C-term diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
mTOR antibody [C3], C-term detects mTOR protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. mTOR antibody [C3], C-term diluted at 1:500.
Application
mTOR antibody detects mTOR protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 100% MeOH for 5 min. Green: mTOR protein stained by mTOR antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Cross-linked ChIP was performed with 293T chromatin extract and 5 μg of either control rabbit IgG or anti-mTOR antibody. The precipitated DNA was detected by PCR with primer set targeting to 45S rDNA promoter .
Application
Immunoprecipitation of mTOR protein from 293T whole cell extracts using 5 μg of mTOR antibody [C3], C-term . Western blot analysis was performed using mTOR antibody [C3], C-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
mTOR antibody immunoprecipitates mTOR protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of mTOR by 3 μg of mTOR antibody 5% SDS-PAGE The immunoprecipitated mTOR protein was detected by mTOR antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Application
mTOR antibody detects FRAP1 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. mTOR antibody dilution: 1:500.
Application
mTOR antibody detects mTOR protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
mTOR antibody detects mTOR protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
mTOR antibody [C3], C-term detects mTOR protein at mitochondria on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. mTOR antibody [C3], C-term diluted at 1:500.| Positive Control | 293T , A431 , HeLa , HepG2 , mouse brain , *Flag-human mTOR transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
