Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [GT6611] diluted at 1:500.Application
mTOR antibody [GT6611] detects mTOR protein by western blot analysis. A Jurkat whole cell lysate/extract BRaji C NCI-H929 whole cell lysate/extract 5 % SDS-PAGE mTOR antibody [GT6611] dilution: 1:1000Application
mTOR antibody [GT6611] detects mTOR protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: mTOR protein stained by mTOR antibody [GT6611] diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
mTOR antibody [GT6611] detects mTOR protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 5 % SDS-PAGE mTOR antibody [GT6611] dilution: 1:500| Product Name | mTOR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT6611] to mTOR (mechanistic target of rapamycin (serine/threonine kinase))The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human mTOR. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | GT6611 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, SheepMouse (99%), Rat (99%), Zebrafish (90%), Sheep (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | RAPT1 FRAP mechanistic target of rapamycin (serine/threonine kinase) FRAP2 MTOR FRAP1 RAFT1 |
|---|---|
| Target | mTOR |
| Cellular Localization | Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side , Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side , Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [GT6611] diluted at 1:500.
Application
mTOR antibody [GT6611] detects mTOR protein by western blot analysis. A Jurkat whole cell lysate/extract BRaji C NCI-H929 whole cell lysate/extract 5 % SDS-PAGE mTOR antibody [GT6611] dilution: 1:1000
Application
mTOR antibody [GT6611] detects mTOR protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: mTOR protein stained by mTOR antibody [GT6611] diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
mTOR antibody [GT6611] detects mTOR protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 5 % SDS-PAGE mTOR antibody [GT6611] dilution: 1:500| Positive Control | Jurkat , Raji , NCI-H929 , 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
