Application
Western blot analysis of extracts from HepG2 cells treated with PMA using CD227/MUC1 (Phospho-Tyr1229) Antibody. The lane on the right is treated with the antigen-specific peptide.Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CD227/MUC1 (Phospho-Tyr1229) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Immunofluorescence staining of methanol-fixed HepG2 cells using CD227/MUC1 (Phospho-Tyr1229) Antibody.| Product Name | MUC1 (phospho Tyr1229) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MUC1 (phospho Tyr1229)This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011] |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 1229 S-P-Y(p)-E-K) derived from Human CD227/MUC1. |
| Modification | p-Tyr1229 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:1000. IHC: 1:50 - 1:100. IF: 1:100 - 1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | mucin 1 cell surface associated PEMT MCKD CD227 MCD KL-6 ADMCKD1 MAM6 ADMCKD MUC-1/X MUC-1/SEC MUC-1 EMA H23AG |
|---|---|
| Target | MUC1 (phospho Tyr1229) |
| Tissue Specificity | The antibody detects endogenous levels of CD227/MUC1 only when phosphorylated at tyrosine 1229. |
Application
Western blot analysis of extracts from HepG2 cells treated with PMA using CD227/MUC1 (Phospho-Tyr1229) Antibody. The lane on the right is treated with the antigen-specific peptide.
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CD227/MUC1 (Phospho-Tyr1229) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Immunofluorescence staining of methanol-fixed HepG2 cells using CD227/MUC1 (Phospho-Tyr1229) Antibody.| Application Notes | WB: 1:500 - 1:1000. IHC: 1:50 - 1:100. IF: 1:100 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
