Application
Baboon Lung- MUC1. Primary Antibody at 1:100 dilution in IHC Blocking Buffer, DAB (brown) staining and Hematoxylin QS (blue) counterstain, 40x magnification on Leica DM4000 microscope. FFPE sertion.| Product Name | MUC1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MUC1MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC-1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands.The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. |
| Immunogen | Synthetic peptide (60 aa long) representing a 20 amino acid repeat sequence from mucin 1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PIP |
| Recommended Starting Dilutions:
For ELISA: Use at a dilution of 1:10,000.
For IHC-P: Use at a dilution of 1:200.
For IP: Use at a dilution of 1:200.
Optimal dilutions should be determined experimentally by the researcher.: |
|
| Species Reactivity | HumanMonkey |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | mucin PEMT MUC1 PUM 4582 158340 P15941 EMA PEM H23AG MAM6 CD227 KL-6 MUC-1 MUC-1/SEC MUC-1/X MUC1/ZD KL 6 MUC 1 MUC 1/X MUC 1/SEC KL6 MUC1/X MUC1/SEC |
|---|---|
| Target | MUC1 |
| Tissue Specificity | The antibody will detect deglycosylated MUCIN1 protein. |
| Cellular Localization | Cell Membrane and Cytoplasmic |
Application
Baboon Lung- MUC1. Primary Antibody at 1:100 dilution in IHC Blocking Buffer, DAB (brown) staining and Hematoxylin QS (blue) counterstain, 40x magnification on Leica DM4000 microscope. FFPE sertion.| Application Notes | Recommended Starting Dilutions:
For ELISA: Use at a dilution of 1:10,000.
For IHC-P: Use at a dilution of 1:200.
For IP: Use at a dilution of 1:200.
Optimal dilutions should be determined experimentally by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Tris, HCl /glycine buffer pH 7.4-7.8; 30% glycerol and 0.5% BSA along with cryo-protective agents; 0.05% sodium azide as preservative. |
