Application
Human Breast Carcinoma stained with anti-MUC-1/EMA/Epithelial Membrane Antigen antibody| Product Name | MUC1 antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MUC1 predilutedMUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC-1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands.The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. |
| Immunogen | Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human), cytoplasmic tail. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| IHC-P: Ready to use. Antigen retrieval is not essential but may optimise staining. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | mucin PEMT MUC1 PUM 4582 158340 P15941 EMA PEM H23AG MAM6 CD227 |
|---|---|
| Target | MUC1 |
| Cellular Localization | Cell Membrane and Cytoplasmic |
Application
Human Breast Carcinoma stained with anti-MUC-1/EMA/Epithelial Membrane Antigen antibody| Positive Control | MCF-7 cells, Breast carcinoma |
|---|---|
| Application Notes | IHC-P: Ready to use. Antigen retrieval is not essential but may optimise staining. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution and store at 4°C. Do not freeze. |
| Storage Buffer | PBS, pH 7.4 containing 1%BSA and 0.1% sodium azide |
