Application
IHC Image using - Detection of MUC5AC by IHC in Stomach| Product Name | Mucin 5AC antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [45M1] to Mucin 5ACMucins are high molecular weight glycoproteins with 80% carbohydrates and 20% core protein. Gastric mucin M1 antigen is found in columnar mucus cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon but not in normal colon. Resurgence of gastric mucin during colonic carcinogenesis is suggestive of either re-expression of the peptide core of gastric mucin in the adult colon or due to changes in the glycosylation pattern of mucin, which expose the hidden M1 antigen. |
| Immunogen | M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le (a-b) patient. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 45M1 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-FIHC-P |
| Immunohistochemistry (formalin fixed paraffin embedded / frozen sections): use at a dilution of 1/50 - 1/75 in an ABC method. Optimal dilutions / concentrations should be determined by the end user. Formalin fixed paraffin embedded tissue sections do not require antigen unmasking prior to immunostaining.: |
|
| Species Reactivity | HumanMouseMonkeyPigRatCatHedgehogRabbit |
| Concentration | 1mg/ml |
| Gene Synonyms | MUC5AC 4586 158373 MUC5 P98088 TBM leB |
|---|---|
| Target | Mucin 5AC |
| Tissue Specificity | This antibody reacts with a 1000 kDa protein and recognizes the peptide core of gastric mucin M1. |
| Cellular Localization | Cytoplasmic, cell surface. |
| Light Chain Type | kappa |
Application
IHC Image using - Detection of MUC5AC by IHC in Stomach| Positive Control | Suitable tissue control sections for Immunohistochemistry:Stomach |
|---|---|
| Application Notes | Immunohistochemistry (formalin fixed paraffin embedded / frozen sections): use at a dilution of 1/50 - 1/75 in an ABC method. Optimal dilutions / concentrations should be determined by the end user. Formalin fixed paraffin embedded tissue sections do not require antigen unmasking prior to immunostaining.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | PBS containing 0.1% sodium azide |
