Mouse monoclonal antibody [1A4+5C5] to Muscle ActinUseful in identifying tumors arising from muscle cells, i.e. leiomyosarcoma as well as rhabdomyosarcoma.
Immunogen
BALB/C mice were injected with N-terminal decapeptide of alpha-smooth muscle actin for 1A4 and with purified rabbit striated muscle actin for 5C5.
Key Feature
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
1A4+5C5
Host Species
Mouse
Tested Applications
IHC-P
IHC-P: 1/25 - 1/50. An incubation period of 30 minutes at room temperature usingf an ABC method is recommended. Optimal dilutions/concentrations should be determined by the end user.:
Species Reactivity
HumanPigRatGuinea PigRabbitSheepSnakeBovine
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Target
Muscle Actin
Cellular Localization
Cytoplasmic
Light Chain Type
kappa
Application
Application
IHC-P analysis of human uterus leiomyoma using Muscle Actin antibody [1A4+5C5].
Positive Control
Skeletal muscle
Application Notes
IHC-P: 1/25 - 1/50. An incubation period of 30 minutes at room temperature usingf an ABC method is recommended. Optimal dilutions/concentrations should be determined by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.