| Product Name | Muscle Actin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3F10] to Muscle ActinThe two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells. |
| Immunogen | Full length purified Actin (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3F10 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-P |
| IF: Use at an assay dependent dilution. IHC-P: Use at a concentration of 1 - 2 μg/ml for 30 min at RT. No special pretreatment is required for immunohistochemical staining of formalin-fixed tissues. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseDogPigRatHorseSilk worm |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | 102610 ASMA NEM1 ACTA Actin NEM2 P68133 58 ACTA1 NEM3 |
|---|---|
| Target | Muscle Actin |
| Tissue Specificity | Reacts with alpha- smooth muscle as well as alpha-skeletal and alpha-cardiac (sarcomeric) isoform of actin. Reacts with tumors arising from smooth muscle (leiomyosarcomas) as well as skeletal muscle (rhabdomyosarcomas). |
| Cellular Localization | Cytoplasmic |
| Light Chain Type | kappa |
| Positive Control | Muscle or sarcoma |
|---|---|
| Application Notes | IF: Use at an assay dependent dilution. IHC-P: Use at a concentration of 1 - 2 μg/ml for 30 min at RT. No special pretreatment is required for immunohistochemical staining of formalin-fixed tissues. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.09% Sodium Azide. Constituents: 40% Glycerol, 0.2% BSA, 10mM PBS. pH 7.4 |
