Application
Myc antibody Immunoprecipitation of Myc tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-Myc antibody . (A) Input, 15ug of HeLa transfected lysates. The precipitated protein was detected by diluted at 1:10000. EasyBlot anti-Rabbit IgG Kit was used in Western blot.Application
Immunofluorescence analysis of paraformaldehyde-fixed Myc tag transfected HeLa, using Myc tag antibody (green) at 1:3000 dilution. Alpha-tubulin filaments were labeled with (red) at 1:2500.Application
Western blot analysis of extracts from untransfected control cells and transfected cells overexpressing Myc tagged protein, using Myc tag antibody. A: Non-transfected 293T lysate (10 µg) B: Transfected 293T lysate (10 µg) (~60 kDa) The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Myc tag antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Rabbit Polyclonal antibody to Myc tag |
| Immunogen | Synthetic peptide corresponding to a region within amino acids 410 and 419 of human c-Myc |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Myc tag antibody |
|---|
Application
Myc antibody Immunoprecipitation of Myc tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-Myc antibody . (A) Input, 15ug of HeLa transfected lysates. The precipitated protein was detected by diluted at 1:10000. EasyBlot anti-Rabbit IgG Kit was used in Western blot.
Application
Immunofluorescence analysis of paraformaldehyde-fixed Myc tag transfected HeLa, using Myc tag antibody (green) at 1:3000 dilution. Alpha-tubulin filaments were labeled with (red) at 1:2500.
Application
Western blot analysis of extracts from untransfected control cells and transfected cells overexpressing Myc tagged protein, using Myc tag antibody. A: Non-transfected 293T lysate (10 µg) B: Transfected 293T lysate (10 µg) (~60 kDa) The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T transfected myc-tag recombinant protein |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
