Application
ELISA analysis of control antigen and antigen using MBP antibody [2H9].Application
FACS analysis of HepG2 cells using MBP antibody [2H9] (green) or a negative control (red).Application
ICC/IF analysis of MSCS cells using MBP antibody [2H9] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).Application
IHC-P analysis of brain tissues using MBP antibody [2H9].Application
IHC-P analysis of cerebellum tissues using MBP antibody [2H9].Application
WB analysis of lysates from (1) untransfected and (2) MBP-hIgGFc transfected HEK293 cells using MBP antibody [2H9].Application
WB analysis of human MBP (a.a. 1-197) recombinant protein using MBP antibody [2H9].| Product Name | Myelin basic protein antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [2H9] to MBPThe protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arra |
| Immunogen | *The immunogen corresponding to a region within amino acids 1 and 197 of human MBP. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2H9 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Target | MBP |
|---|---|
| Molecular Weight(MW) | 33 kDa (note) |
Application
ELISA analysis of control antigen and antigen using MBP antibody [2H9].
Application
FACS analysis of HepG2 cells using MBP antibody [2H9] (green) or a negative control (red).
Application
ICC/IF analysis of MSCS cells using MBP antibody [2H9] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).
Application
IHC-P analysis of brain tissues using MBP antibody [2H9].
Application
IHC-P analysis of cerebellum tissues using MBP antibody [2H9].
Application
WB analysis of lysates from (1) untransfected and (2) MBP-hIgGFc transfected HEK293 cells using MBP antibody [2H9].
Application
WB analysis of human MBP (a.a. 1-197) recombinant protein using MBP antibody [2H9].| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
