Application
FACS analysis of human peripheral blood granulocytes using Myeloperoxidase antibody [2C7].Application
IHC-P analysis of human bone marrow tissue using Myeloperoxidase antibody [2C7].| Product Name | Myeloperoxidase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2C7] to MyeloperoxidaseHuman myeloperoxidase (MPO) is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55kD) and two light chains (15kD), the heavy chains being linked by a disulphide bond. |
| Immunogen | Human myeloperoxidase |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2C7 |
| Host Species | Mouse |
| Tested Applications | FACSIHCIHC-FIHC-P |
| For FACS: Use at a dilution of 1:50 - 1:100. For IHC-Fr: Use at a dilution of 1:1,000 - 1:5,000. For IHC-P: Use at a dilution of 1:500 - 1:1,000. Optimal concentrations/dilutions should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | Myeloperoxidase |
|---|---|
| Tissue Specificity | recognises native MPO in Western blots, and the heavy chain following boiling of the sample. The antibody also recognises recombinant MPO in Western blots and weakly in ELISA. The antibody may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeablisation. |
Application
FACS analysis of human peripheral blood granulocytes using Myeloperoxidase antibody [2C7].
Application
IHC-P analysis of human bone marrow tissue using Myeloperoxidase antibody [2C7].| Positive Control | Bone marrow |
|---|---|
| Application Notes | For FACS: Use at a dilution of 1:50 - 1:100. For IHC-Fr: Use at a dilution of 1:1,000 - 1:5,000. For IHC-P: Use at a dilution of 1:500 - 1:1,000. Optimal concentrations/dilutions should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4, containing 0.09% sodium azide |
