Application
Immunohistochemical analysis of paraffin-embedded human adenocarcinoma, using MYH9 antibody at 1:500 dilution.Application
MYH9 antibody [N1], N-term detects MYH9 protein by Western blot analysis. A. 30 µg U87-MG whole cell lysate/extract B. 30 µg SK-N-SH whole cell lysate/extract C. 30 µg IMR32 whole cell lysate/extract D. 30 µg SK-N-AS whole cell lysate/extract 5 % SDS-PAGE MYH9 antibody [N1], N-term dilution: 1:1000Application
MYH9 antibody [N1], N-term detects MYH9 protein by Western blot analysis. A. 30 µg NT2D1 whole cell lysate/extract B. 30 µg PC-3 whole cell lysate/extract 5 % SDS-PAGE MYH9 antibody [N1], N-term dilution: 1:1000Application
Immunoprecipitation of MYH9 protein from HeLa whole cell extracts using 5 μg of MYH9 antibody [N1], N-term . Western blot analysis was performed using MYH9 antibody [N1], N-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | MYH9 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MYH9 (myosin, heavy chain 9, non-muscle)This gene encodes a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain. The protein is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 are the cause of non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human MYH9. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Dog, Chicken, BovineMouse (98%), Rat (97%), Xenopus laevis (92%), Dog (99%), Chicken (96%), Bovine (99%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DFNA17 antibody EPSTS antibody FTNS antibody MGC104539 antibody MHA antibody NMHC-II-A antibody NMMHCA antibody MYH9 antibody myosin heavy chain 9 antibody non-muscle myosin heavy chain IIa antibody non-muscle myosin heavy chain A antibody myosi |
|---|---|
| Molecular Weight(MW) | 227 kDa (note) |
Application
Immunohistochemical analysis of paraffin-embedded human adenocarcinoma, using MYH9 antibody at 1:500 dilution.
Application
MYH9 antibody [N1], N-term detects MYH9 protein by Western blot analysis. A. 30 µg U87-MG whole cell lysate/extract B. 30 µg SK-N-SH whole cell lysate/extract C. 30 µg IMR32 whole cell lysate/extract D. 30 µg SK-N-AS whole cell lysate/extract 5 % SDS-PAGE MYH9 antibody [N1], N-term dilution: 1:1000
Application
MYH9 antibody [N1], N-term detects MYH9 protein by Western blot analysis. A. 30 µg NT2D1 whole cell lysate/extract B. 30 µg PC-3 whole cell lysate/extract 5 % SDS-PAGE MYH9 antibody [N1], N-term dilution: 1:1000
Application
Immunoprecipitation of MYH9 protein from HeLa whole cell extracts using 5 μg of MYH9 antibody [N1], N-term . Western blot analysis was performed using MYH9 antibody [N1], N-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | U87-MG , SK-N-SH , IMR32 , SK-N-AS , NT2D1 , PC-3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
