Application
Immunoprecipitation of MYH9 protein from HeLa whole cell extracts using 5 μg of MYH9 antibody [N1-2], N-term . Western blot analysis was performed using MYH9 antibody [N1-2], N-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
MYH9 antibody [N1-2], N-term detects MYH9 protein at cytoplasm in mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. MYH9 antibody [N1-2], N-term diluted at 1:400.Application
MYH9 antibody [N1-2], N-term detects MYH9 protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. MYH9 antibody [N1-2], N-term diluted at 1:500.Application
MYH9 antibody [N1-2], N-term detects MYH9 protein by western blot analysis. A. 30 µg Rat2 whole cell lysate/extract 5% SDS-PAGE MYH9 antibody [N1-2], N-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MYH9 antibody at 1:200 dilution.Application
MYH9 antibody [N1-2], N-term detects MYH9 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 5% SDS-PAGE MYH9 antibody [N1-2], N-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
MYH9 antibody [N1-2], N-term detects MYH9 protein by western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract B. 30 µg BCL-1 whole cell lysate/extract C. 30 µg Raw264.7 whole cell lysate/extract D. 30 µg C2C12 whole cell lysate/extract 5% SDS-PAGE MYH9 antibody [N1-2], N-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | MYH9 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MYH9 (myosin, heavy chain 9, non-muscle)This gene encodes a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain. The protein is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 are the cause of non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human MYH9. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FIHC-PIPWB |
| WB:1:500~1:1000 IP:1:100~1:500 ICC/IF:1:100~1:500 IHC:1:50~1:500 |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Dog, Chicken, BovineXenopus laevis (89%), Dog (99%), Chicken (94%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DFNA17 antibody EPSTS antibody FTNS antibody MGC104539 antibody MHA antibody NMHC-II-A antibody NMMHCA antibody MYH9 antibody myosin heavy chain 9 antibody non-muscle myosin heavy chain IIa antibody non-muscle myosin heavy chain A antibody myosi |
|---|---|
| Molecular Weight(MW) | 227 kDa (note) (2) |
Application
Immunoprecipitation of MYH9 protein from HeLa whole cell extracts using 5 μg of MYH9 antibody [N1-2], N-term . Western blot analysis was performed using MYH9 antibody [N1-2], N-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
MYH9 antibody [N1-2], N-term detects MYH9 protein at cytoplasm in mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. MYH9 antibody [N1-2], N-term diluted at 1:400.
Application
MYH9 antibody [N1-2], N-term detects MYH9 protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. MYH9 antibody [N1-2], N-term diluted at 1:500.
Application
MYH9 antibody [N1-2], N-term detects MYH9 protein by western blot analysis. A. 30 µg Rat2 whole cell lysate/extract 5% SDS-PAGE MYH9 antibody [N1-2], N-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MYH9 antibody at 1:200 dilution.
Application
MYH9 antibody [N1-2], N-term detects MYH9 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 5% SDS-PAGE MYH9 antibody [N1-2], N-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
MYH9 antibody [N1-2], N-term detects MYH9 protein by western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract B. 30 µg BCL-1 whole cell lysate/extract C. 30 µg Raw264.7 whole cell lysate/extract D. 30 µg C2C12 whole cell lysate/extract 5% SDS-PAGE MYH9 antibody [N1-2], N-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , HeLa , A375 , NIH-3T3 , BCL-1 , C2C12 , Rat2 |
|---|---|
| Application Notes | WB:1:500~1:1000 IP:1:100~1:500 ICC/IF:1:100~1:500 IHC:1:50~1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
