Mouse monoclonal antibody [AT4E8] to MYL4Myosin is a hexameric ATPase cellular motor protein. It is composed of two myosin heavy chains, two nonphosphorylatable myosin alkali light chains, and two phosphorylatable myosin regulatory light chains. This gene encodes a myosin alkali light chain that is found in embryonic muscle and adult atria. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Immunogen
Recombinant human MYL4 (1-197aa) purified from E. coli
Key Feature
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
AT4E8
Host Species
Mouse
Tested Applications
ELISAWB
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.:
Species Reactivity
Human
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
WB analysis of HeLa lysate (40ug) using MYL4 antibody at a dilution of 1:1,000.
Application Notes
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.:
Additional Information
Form
Liquid
Storage Instructions
Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.