Application
Immunofluorescence staining of methanol-fixed U87 cells using MLC2 (Phospho-Tyr118) Antibody.Application
U87 cells were synchronized in I (interphase) and C (Cytokinesis) respectively, then were harvested for immunoblotting.| Product Name | MYL9 (phospho Tyr118) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MYL9 (phospho Tyr118)Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 118 (A-D-Y(p)-V-R) derived from Human MLC2. |
| Modification | p-Tyr118 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWBIB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MLC-2C MYL9 LC20 MYRL2 myosin light chain 9 regulatory MRLC1 MLC2 |
|---|---|
| Target | MYL9 (phospho Tyr118) |
| Tissue Specificity | The antibody detects endogenous level of MLC2 only when phosphorylated at tyrosine 118. |
Application
Immunofluorescence staining of methanol-fixed U87 cells using MLC2 (Phospho-Tyr118) Antibody.
Application
U87 cells were synchronized in I (interphase) and C (Cytokinesis) respectively, then were harvested for immunoblotting.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C |
| Storage Buffer | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
