Application
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MYLIP Antibody. The picture on the right is treated with the synthesized peptide.Application
Western blot analysis of extracts from HeLa/Jurkat cells, using MYLIP Antibody. The lane on the right is treated with the synthesized peptide.| Product Name | MYLIP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MYLIPE3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MYLIP. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCIHC-PWB |
| IHC: 1:50~1:100 : |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Myosin regulatory light chaininteracting protein EC6.3.2. EC 6.3.2. EC 6.3.2.- MIR E3 ubiquitin-protein ligase MYLIP Myosin regulatory light chain-interacting protein MYLIP E3 ubiquitinprotein ligase MYLIP |
|---|---|
| Target | MYLIP |
| Molecular Weight(MW) | 49 kDa (note) |
| Tissue Specificity | MYLIP Antibody detects endogenous levels of total MYLIP protein. |
Application
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MYLIP Antibody. The picture on the right is treated with the synthesized peptide.
Application
Western blot analysis of extracts from HeLa/Jurkat cells, using MYLIP Antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | IHC: 1:50~1:100 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
