| Product Name | MyoD1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [5.2F] to MyoD1This gene product belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It is involved in muscle cell differentiation, and is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis. Myo D1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of Myo D1 is seen in ectomesenchymoma and a subset of Wilm's tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing's sarcomas and alveolar soft part sarcomas. |
| Immunogen | Recombinant full length protein (Mouse). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 5.2F |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-P |
| IHC: This antibody may be diluted to a titer of 1:20-1:50 in an ABC method. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded alltissue sections require heat mediated antigen retrieval with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Gene Synonyms | AI503393 MyoD Myod-1 17927 MYF3 MyoD1 Myod 1 |
|---|---|
| Target | MyoD1 |
| Tissue Specificity | This antibody is specific to an epitope of amino acids 3-56 in the N-terminus of the 45 kDa mouse MyoD1 protein. This antibody does not react with myogenin, Myf5 or Myf6. MyoD1 stains the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue but is expressed strongly in the tumor cell nuclei of rhabdomyosarcomas. |
| Application Notes | IHC: This antibody may be diluted to a titer of 1:20-1:50 in an ABC method. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded alltissue sections require heat mediated antigen retrieval with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | Tissue culture supernatant with sodium azide |
