Application
Myogenin antibody detects Myogenin protein by western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Myogenin antibody at a dilution of 1:1000.| Product Name | Myogenin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Myogenin (myogenin (myogenic factor 4))Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Myogenin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Rabbit, Sheep, BovineMouse (95%), Rat (95%), Pig (95%), Rabbit (96%), Sheep (92%), Bovine (92%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | bHLHc3 MYOG MYF4 myogenin (myogenic factor 4) myf-4 |
|---|---|
| Target | Myogenin |
| Molecular Weight(MW) | 25 kDa (note) |
| Cellular Localization | Nucleus |
Application
Myogenin antibody detects Myogenin protein by western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Myogenin antibody at a dilution of 1:1000.| Positive Control | RD |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
