Application
Immunofluorescence staining of methanol-fixed Hela cells using Myosin Light Chain 2 Antibody .Application
Western blot analysis of extracts from K562 cells using Myosin Light Chain 2 Antibody and the same antibody preincubated with blocking peptide.| Product Name | Myosin Light Chain 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Myosin Light Chain 2Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion |
| Immunogen | Peptide sequence around aa.16~20 (A-T-S-N-V) derived from Human Myosin Light Chain 2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MLCB MRCL3 MRLC3 MYL2B MYL12A myosin regulatory light chain MRLC3 MLC-2B myosin regulatory light chain 12A myosin RLC myosin regulatory light chain MRCL3 myosin regulatory light chain 2 nonsarcomeric myosin light polypeptide regulatory non- |
|---|---|
| Target | Myosin Light Chain 2 |
| Molecular Weight(MW) | 18 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using Myosin Light Chain 2 Antibody .
Application
Western blot analysis of extracts from K562 cells using Myosin Light Chain 2 Antibody and the same antibody preincubated with blocking peptide.| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
