| Product Name | Myosin skeletal fast antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Mouse Antibody to Skeletal Myosin ( Fast )Myosin is a 480 kD protein that interacts with actin in muscle and non muscle cells (8). Conventional myosin contains two identical heavy chains (MHC - 200kD each) and four light chains (MLC-15-26 kD). Conventional myosin molecules consist of two major regions: tail (rod) and globular heads; they aggregate into filaments through the tail region and interact with actin and with adenosine triphosphate (ATP) through the head region. Multiple forms of myosin heavy chains exist in skeletal, smooth and cardiac muscle and in non- muscle tissues. A spectrum of skeletal muscle fiber types is found in adult skeletal muscles. The two major skeletal muscle fiber types are type I (slow- twitch) and type II (fast-twitch). Skeletal muscle type II fibers can be further subdivided into types IIa (fast red) and IIb (fast white). |
| Immunogen | Rabbit muscle myosin |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MY32 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-P |
| This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues.: |
|
| Species Reactivity | MousePigRatGuinea PigRabbit |
| Concentration | 1mg/ml |
| Target | Myosin skeletal fast |
|---|---|
| Tissue Specificity | This antibody recognizes heavy chain of human skeletal myosin. It does not stain cardiac or smooth muscle myosin. This antibody stains the fast twitch (type II) isomyosin molecule. |
| Cellular Localization | Cytoplasmic |
| Positive Control | Skeletal muscle |
|---|---|
| Application Notes | This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 0.09% sodium azide |
