| Product Name | NALP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to NALP1Death Effector Filament-forming Ced 4 like Apoptosis Protein (DEFCAP) is a novel apoptotic protein of the mammalian Ced 4 family. It has two isoforms: DEFCAP-Land DEFCAP-S. The deduced amino acid sequences of these two alternately spliced isoforms only differ by 44amino acids at the C-terminus. DEFCAP contains a caspase recruitment domain (CARD), a domain that with the proper stimulation can recruit caspases to form cytoplasmic structures called death effector filaments, which in turn initiate the apoptotic signaling pathway. |
| Immunogen | Synthetic peptide WEKGSKKGLLPLSScorresponding to C-term of human DEFCAP in both theDEFCAP-L and ?S isoforms. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| Immunoprecipitation: use at a concentration of 3.0 - 5.0 μg / extract from 10^7 cells. Western Blotting: use at a concentration of 0.5 - 2.0 μg/ml. ELISA: use at a concentration of 0.1 - 1.0 μg/ml. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of approximately 165 kDa in rat brain tissue lysate, which corresponds to the predicted molecular weight of DEFCAP. Sample wasboiled prior to subjecting on 7.5% SDS-PAGE. An ~80 kDa band was also observed in this blot.: |
|
| Species Reactivity | HumanMonkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 606636 22861 CARD7 NALP1 Q9C000 KIAA0926 PP1044 DKFZp586O1822 DEFCAPL/S DEFCAP DEFCAP-L/S DEFCAP L/S |
|---|---|
| Target | DEFCAP |
| Tissue Specificity | This antibody reacts predominantly with DEFCAP. |
| Application Notes | Immunoprecipitation: use at a concentration of 3.0 - 5.0 μg / extract from 10^7 cells. Western Blotting: use at a concentration of 0.5 - 2.0 μg/ml. ELISA: use at a concentration of 0.1 - 1.0 μg/ml. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of approximately 165 kDa in rat brain tissue lysate, which corresponds to the predicted molecular weight of DEFCAP. Sample wasboiled prior to subjecting on 7.5% SDS-PAGE. An ~80 kDa band was also observed in this blot.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS with 0.05% sodium azide |
