| Product Name | NAP2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to NAP2NAP2 is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and sythesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells. |
| Immunogen | Highly pure (>98%) recombinant hNAP-2 (human Neutrophil Activating Protein-2). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISANeutWB |
| Neutralization:To yield one-half maximal inhibition [ND 50 ] of the biological activity of hNAP-2 (50 ng/ml), a concentration of 1.75 - 3.0 μg/ml of this antibody is required. ELISA:To detect hNAP-2 by direct ELISA (using 100μl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hNAP-2. Western Blot:To detect hNAP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | B TG1 Beta TG Beta thromboglobulin Chemokine (C X C motif) ligand 7 Connective tissue activating peptide III CTAP3 CTAPIII CXC chemokine ligand 7 CXCL7 LA PF4 LDGF Low affinity platelet factor IV MDGF NAP 2 L1 Neutrophil activating peptide 2 |
|---|---|
| Target | NAP2 |
| Cellular Localization | Secreted |
| Application Notes | Neutralization:To yield one-half maximal inhibition [ND 50 ] of the biological activity of hNAP-2 (50 ng/ml), a concentration of 1.75 - 3.0 μg/ml of this antibody is required. ELISA:To detect hNAP-2 by direct ELISA (using 100μl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hNAP-2. Western Blot:To detect hNAP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | PBS, pH 7.4, no preservative, sterile filtered |
