Application
Sample (50 ug of whole cell lysate) A: mouse muscle 7.5% SDS PAGE diluted at 1:1000Application
NARF antibody detects NARF protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NARF protein stained by NARF antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using NARF antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 7.5% SDS PAGE diluted at 1:1000| Product Name | NARF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NARF (nuclear prelamin A recognition factor)Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NARF. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (93%), Bovine (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp434G0420 antibody FLJ10067 antibody IOP2 antibody NARF antibody iron-only hydrogenase-like protein 2 antibody prenyl-dependent prelamin A binding protein antibody nuclear prelamin A recognition factor antibody |
|---|---|
| Molecular Weight(MW) | 51 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
Sample (50 ug of whole cell lysate) A: mouse muscle 7.5% SDS PAGE diluted at 1:1000
Application
NARF antibody detects NARF protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NARF protein stained by NARF antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using NARF antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 7.5% SDS PAGE diluted at 1:1000| Positive Control | A549 , H1299 , HCT116 , MCF-7 , mouse muscle |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
