Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NARF antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: A549 B: MCF-7 7.5% SDS PAGE diluted at 1:1000Application
NARF antibody [N3C2], Internal detects NARF protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. NARF antibody [N3C2], Internal dilution: 1:250.| Product Name | NARF antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NARF (nuclear prelamin A recognition factor)Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NARF. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp434G0420 antibody FLJ10067 antibody IOP2 antibody NARF antibody iron-only hydrogenase-like protein 2 antibody prenyl-dependent prelamin A binding protein antibody nuclear prelamin A recognition factor antibody |
|---|---|
| Molecular Weight(MW) | 51 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NARF antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: A549 B: MCF-7 7.5% SDS PAGE diluted at 1:1000
Application
NARF antibody [N3C2], Internal detects NARF protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. NARF antibody [N3C2], Internal dilution: 1:250.| Positive Control | A549 , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
