Application
Western Blot: NCOA2 Antibody - Detection of Human SRC2 on HeLa whole cell lysate using .| Product Name | NCOA2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to NCOA2Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcriptional activity of the nuclear receptor with which they associate. One such coactivator is nuclear receptor coactivator 2. |
| Immunogen | Immunogen was a synthetic peptide, which represented a portion of human nuclear receptor coactivator 2 encoded near the 3 prime end of exon 13 (LocusLink ID 10499). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| IP: Use at a concentration of 1 - 4 µg/mg of lysate. WB: Use at a dilution of 1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 175 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseXenopus |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | TIF2 GRIP1 NCoA2 601993 Q15596 NCoA-2 10499 NCoA 2 |
|---|---|
| Target | NCOA2 |
| Cellular Localization | Nuclear |
Application
Western Blot: NCOA2 Antibody - Detection of Human SRC2 on HeLa whole cell lysate using .| Positive Control | Nuclear extract from HeLa cells |
|---|---|
| Application Notes | IP: Use at a concentration of 1 - 4 µg/mg of lysate. WB: Use at a dilution of 1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 175 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: Tris-citrate/phosphate. pH 7-8 |
