Application
Western blot analysis of Nectin-3 expression in lysate from rat kidney (Lane 1) and 3T3 cells (Lane 2).| Product Name | Nectin-3/CD113 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Nectin-3/CD113Nectin is a cell-adhesion molecule that belongs to the immunoglobilin superfamily and is also known as the poliovirus receptor-related protein or PPR. Nectin family consists of Nectin 1 which expressed abundantly in the brain and Nectin 2 and 3 which are ubiquitously expressed in cells where cadherin are not expressed. Nectin-3 or PPR3 is a transmembrane protein that has three splicing variants: Nectin3alpha , Nectin 3beta and Nectin 3g. Nectin-3 is mainly expressed in the testis and placental tissues and interacts in vivo with both long and short isoforms of afadin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~ 83 kDa Nectin-3 of mouse and rat origins. Reactivity to other species has not been tested.: |
|
| Species Reactivity | MouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PRR-3 PVRL-3 PRR3 PVRL3 PVRL 3 Poliovirus receptorrelated protein 3 CDw113 CD113 Nectin3 Nectin 3 Poliovirus receptor-related protein 3 Poliovirus receptorrelated protein3 PRR 3 |
|---|---|
| Target | Nectin-3/CD113 |
| Tissue Specificity | This antibody is specific for Nectin-3/CD113. |
Application
Western blot analysis of Nectin-3 expression in lysate from rat kidney (Lane 1) and 3T3 cells (Lane 2).| Application Notes | Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~ 83 kDa Nectin-3 of mouse and rat origins. Reactivity to other species has not been tested.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, 0.01% thimerosal |
