Application
Detection of human NEK2 protein using GeneTex NEK2 1B6 monoclonal antibody in Jurkat whole cell extract.| Product Name | NEK2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [1B6] to NEK2NEK2 is closely related in its catalytic domain to the serine/threonine protein kinase NIMA of Aspergillus nidulans which is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the Nek2 protein is almost undetectable during G1 but accumulates progressively throughout S, reaching maximal levels in late G2. These observations demonstrate that NEK2 resembles Aspergillus NIMA, both in its catalytic domain, and cell cycle-dependent expression. Recombinant NEK2 is active as a serine/threonine-specific protein kinase and may undergo autophosphorylation. Both human NEK2 and fungal NIMA phosphorylate a similar, but not identical, set of proteins and synthetic peptides. NEK2 is shown to be expressed most abundantly in adult testis and there is increasing evidence that NEK2 is abnormally expressed in a wide variety of human cancers. Both isoforms are expressed in pheripheral T cells and many other transformed cell types. |
| Immunogen | Synthetic peptide Ð Immunogen sequence information is commercially sensitive |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | 1B6 |
| Host Species | Mouse |
| Tested Applications | WB |
| For WB: Use at a dilution of 1:500-1:1,000. It detects both isoforms 1 & 2 in Jurkat lysate - see image online. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EC 2.7.11.1 - HsPK21 - NLK - NLK1 - NEK - NEK2 -4751 Serine threonine protein kinase Nek2 antibody HsPK 21 antibody HsPK21 antibody NEK 2 antibody NEK 2A antibody NEK2A antibody Never in mitosis gene A related kinase 2 antibody NIMA (never in mitosis gene |
|---|---|
| Target | NEK2 |
| Molecular Weight(MW) | 52 kDa (note) |
| Cellular Localization | Nuclear. Localizes at the centromere. Isoform 1 has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end missing in isoform 2 which is predominantly cytoplasmic. |
Application
Detection of human NEK2 protein using GeneTex NEK2 1B6 monoclonal antibody in Jurkat whole cell extract.| Positive Control | Jurkat cell lysate |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:500-1:1,000. It detects both isoforms 1 & 2 in Jurkat lysate - see image online. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing no preservatives |
