Application
Western blot analysis of NENF in human kidney tissue lysate with NENF antibody at (A) 1 and (B) 2 ug/ml.Application
Immunohistochemistry of NENF in rat kidney tissue with NENF antibody at 10 ug/ml.| Product Name | NENF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to NENFNENF is a secreted protein that is expressed in neurons but not glial cells of the brain. This protein has neurotrophic activity in primary cultured mouse neurons but not mitogenic activity in primary cultured mouse astrocytes. NENF activated mitogen-activated protein (MAP) and phosphotidylinositol-3 kinase pathways and could be inhibited by pertussis toxin but not receptor tyrosine kinases, suggesting that NENF acts through a Gi/Go-protein-coupled receptor. Despite its predicted molecular weight of ~20kDa, NENF is observed at 37kDa in SDS-PAGE. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | SPUF CIR2 neudesin SCIRP10 NENF Neuron derived neurotrophic factor |
|---|---|
| Target | NENF |
| Tissue Specificity | This antibody is specific for NENF |
Application
Western blot analysis of NENF in human kidney tissue lysate with NENF antibody at (A) 1 and (B) 2 ug/ml.
Application
Immunohistochemistry of NENF in rat kidney tissue with NENF antibody at 10 ug/ml.| Positive Control | Blocking Peptide: Cat.No. GTX85028-PEP - NENF Peptide Lysates: Cat. No. GTX27923 - Human Small Intestine Tissue Lysate |
|---|---|
| Application Notes | IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Antibody is supplied in PBS containing 0.02% sodium azide |
