Application
Western blot analysis of CPG2 in adult rat brain fractions and whole cell lysates from mock or CPG2 transfected HEK293T cells. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with CPG2 antibody [6A] at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a HRP-conjugated secondary antibody. Membranes were washed and chemiluminescent detection was performed.| Product Name | Nesprin 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [6A] to Nesprin 1 |
| Immunogen | Recombinant protein containing residues RKLESTLTGLEQSRERQERR |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6A |
| Host Species | Mouse |
| Tested Applications | WB |
| WB: 1:1000 Optimal dilution / concentration should be determined by the end user.: |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | spectrin repeat containing nuclear envelope 1 Syne1 Myne-1 |
|---|---|
| Target | Nesprin 1 |
Application
Western blot analysis of CPG2 in adult rat brain fractions and whole cell lysates from mock or CPG2 transfected HEK293T cells. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with CPG2 antibody [6A] at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a HRP-conjugated secondary antibody. Membranes were washed and chemiluminescent detection was performed.| Application Notes | WB: 1:1000 Optimal dilution / concentration should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20°C |
| Storage Buffer | PBS containing 1 mg/ml BSA and 0.05 % sodium azide |
