Rabbit polyclonal antibody to Nesprin 3Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system.
Immunogen
The antiserum was produced against synthesized peptide derived from human C14orf49.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ELISA: 1:40000Optimal dilutions should be determined experimentally by the end user.:
Species Reactivity
HumanMouse
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
Nesprin 3 C14orf49
Target
C14orf49
Molecular Weight(MW)
112 kDa (note)
Tissue Specificity
C14orf49 Antibody detects endogenous levels of total C14orf49 protein.
Cellular Localization
Located at the rough reticulum endoplasmic (RER) in some cells.
Application
Application
Western blot analysis of extracts from Jurkat cells, using C14orf49 Antibody. The lane on the right is treated with the synthesized peptide.
Application Notes
ELISA: 1:40000Optimal dilutions should be determined experimentally by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Store at -20 degrees Celcius. Product is stable for at least 1 year.
Storage Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.