Application
Visualization of Nestin in PC-3 cells using Nestin Antibody [10C2] . Courtesy of Dolnick Lab, Roswell Park Cancer Institute.Application
Detection of human nestin using Nestin Antibody [10C2] . Lane 1: 5 ug total rat brain protein, lane 2: 5 ug human CNS progenitor cell protein.Application
FACS staining of NTERA-2 cells using Nestin Antibody [10C2] at a 1:50 dilution detected using Dylight-488 conjugated goat anti-mouse IgG secondary antibody.Application
Immunocytochemistry/Immunofluorescence: Nestin Antibody (10C2) [ - HeLa cells stained (Green) detected with DyLight Fluor 488 conjugated anti-rabbit IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue)| Product Name | Nestin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody to Nestin [10C2] - Neural Stem Cell MarkerNestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor. |
| Immunogen | A fragment of the human nestin protein corresponding to residues 1464-1614. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 10C2 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHC-FIHC-PWB |
| Flow cytometry / FACS analysis 1:50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FLJ21841 10763 Nbla00170 P48681 NES Nestin 600915 |
|---|---|
| Target | Nestin |
| Tissue Specificity | This antibody is specific for human nestin. |
Application
Visualization of Nestin in PC-3 cells using Nestin Antibody [10C2] . Courtesy of Dolnick Lab, Roswell Park Cancer Institute.
Application
Detection of human nestin using Nestin Antibody [10C2] . Lane 1: 5 ug total rat brain protein, lane 2: 5 ug human CNS progenitor cell protein.
Application
FACS staining of NTERA-2 cells using Nestin Antibody [10C2] at a 1:50 dilution detected using Dylight-488 conjugated goat anti-mouse IgG secondary antibody.
Application
Immunocytochemistry/Immunofluorescence: Nestin Antibody (10C2) [ - HeLa cells stained (Green) detected with DyLight Fluor 488 conjugated anti-rabbit IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue)| Application Notes | Flow cytometry / FACS analysis 1:50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | 0.1 ml of protein G purified ascites. Contains 0.1% sodium azide. |
