Application
Western blot analysis of Heregulin using| Product Name | Neuregulin-1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Neuregulin-1Heregulin (HRG) or Neu Differentiation Factor (NDF) binds to c-erbB-3 and cerbB-4 receptors on mammary and neuronal cells with low and high affinities, respectively. HRG/NDF exists in several isoforms, which are classified in two groups, a and b, that differ in their EGF-like domain and in receptor binding affinity. |
| Immunogen | Synthetic peptide from the EGF-like domain of human heregulin |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | BlockingWB |
| For Blocking - Use at 50 μg/ml culture medium. For WB: Use at a concentration of 5-10 μg/ml for 2 hrs at RT. Not suitable for use in IHC. Optimal dilutions/concentrations should be determined by the end user: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Neuregulin GGF Heregulin 1 Heregulin1 NDF |
|---|---|
| Target | Neuregulin-1 |
| Tissue Specificity | Reacts weakly with alpha-HRG and strongly with beta-HRG. Does not inhibit the binding of HRG to ErbB receptors but it efficiently blocks the HRG-induced proliferation and differentiation of cultured human mammary cells. |
Application
Western blot analysis of Heregulin using| Positive Control | HRGbeta1 recombinant protein, LS174T cells |
|---|---|
| Application Notes | For Blocking - Use at 50 μg/ml culture medium. For WB: Use at a concentration of 5-10 μg/ml for 2 hrs at RT. Not suitable for use in IHC. Optimal dilutions/concentrations should be determined by the end user: |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, no preservative added. |
