Application
Cell lysates of 293T (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human NRG1 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Product Name | Neuregulin-1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [k1G13] to Neuregulin-1Neuregulin1 (NRG1) is one of four proteins in the neuregulin family that act on the Epidermal growth factor receptor (EGFR) family of receptors. NRG1 was originally identified as a 44 kDa glycoprotein that interacts with the ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. In many brain areas, NRG1 is expressed at synaptic regions and its processing can be regulated by neuronal activity. This variant (HRG-beta3) of NRG1, along with HRG-alpha, beta1 and beta2 variants, was identified in various normal tissues and cancer cell lines. |
| Immunogen | Recombinant human NRG1 isoform HRG-beta3 (20-241aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | k1G13 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot: Use at 1:1,000 ~ 2,000.Optimal working concentrations should be determined experimentally by the end user.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HRG neuregulin 1 isoform HRG-beta3 NRG1 neuregulin1 GGF neuregulin1 isoform HRGbeta3 neuregulin 1 isoform HRGbeta3 NDF neuregulin 1 neuregulin 1 isoform HRG beta3 |
|---|---|
| Target | Neuregulin-1 |
| Cellular Localization | Does not seem to be active. May be nuclear. Has a signal peptide. May possess an internal uncleaved signal sequence. |
| Light Chain Type | kappa |
Application
Cell lysates of 293T (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human NRG1 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Application Notes | Western Blot: Use at 1:1,000 ~ 2,000.Optimal working concentrations should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
