Application
Immunofluorescent analysis of Neurofibromin in HeLa cells. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with Neurofibromin antibody [McNFn27b] at a dilution of 1:20 overnight at 4°C, washed with PBS and incubated with a proper secondary antibody. Neurofibromin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.Application
Immunohistochemistry was performed on cancer biopsies of deparaffinized human breast carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed at a dilution of 1:20 with or without Neurofibromin antibody [McNFn27b] overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.| Product Name | Neurofibromin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [McNFn27b] to NeurofibrominThis gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | Synthetic peptide corresponding to the N-terminal residues 27-41 of human neurofibromin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | McNFn27b |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIHC-PWB |
| ICC/IF: 1:10 - 1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | VRNF WSS NFNS NF1 neurofibromin 1 |
|---|---|
| Target | Neurofibromin |
Application
Immunofluorescent analysis of Neurofibromin in HeLa cells. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with Neurofibromin antibody [McNFn27b] at a dilution of 1:20 overnight at 4°C, washed with PBS and incubated with a proper secondary antibody. Neurofibromin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Application
Immunohistochemistry was performed on cancer biopsies of deparaffinized human breast carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed at a dilution of 1:20 with or without Neurofibromin antibody [McNFn27b] overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.| Application Notes | ICC/IF: 1:10 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20° C, Avoid Freeze/Thaw Cycles |
| Storage Buffer | PBS containing 1 mg/ml BSA and 0.05 % sodium azide |
