Application
NFAT1 antibody immunoprecipitates NFAT1 protein in IP experiments. IP samples: Raji whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of NFAT1 protein by 4 μg NFAT1 antibody 7.5 % SDS-PAGE The immunoprecipitated NFAT1 protein was detected by NFAT1 antibody diluted at 1:500.Application
NFAT1 antibody detects NFATC2 protein by western blot analysis. A. 30 µg BCL-1 whole cell lysate/extract B. 30 µg Raw264.7 whole cell lysate/extract 7.5% SDS-PAGE NFAT1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
NFAT1 antibody detects NFAT1 protein at cytoplasm by immunofluorescent analysis. Sample: RAW264.7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFAT1 protein stained by NFAT1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
NFAT1 antibody detects NFATC2 protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract 7.5% SDS-PAGE NFAT1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | NFAT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NFAT1 (nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2)This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse NFAT1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | RatRat (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | NFAT1 antibody NFATP antibody NFATC2 antibody nuclear factor of activated T-cells preexisting component antibody NFAT transcription complex preexisting component antibody T-cell transcription factor NFAT1 antibody preexisting nuclear factor of acti |
|---|---|
| Molecular Weight(MW) | 100 kDa (note) (6) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
NFAT1 antibody immunoprecipitates NFAT1 protein in IP experiments. IP samples: Raji whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of NFAT1 protein by 4 μg NFAT1 antibody 7.5 % SDS-PAGE The immunoprecipitated NFAT1 protein was detected by NFAT1 antibody diluted at 1:500.
Application
NFAT1 antibody detects NFATC2 protein by western blot analysis. A. 30 µg BCL-1 whole cell lysate/extract B. 30 µg Raw264.7 whole cell lysate/extract 7.5% SDS-PAGE NFAT1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
NFAT1 antibody detects NFAT1 protein at cytoplasm by immunofluorescent analysis. Sample: RAW264.7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFAT1 protein stained by NFAT1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
NFAT1 antibody detects NFATC2 protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract 7.5% SDS-PAGE NFAT1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Juarkat , Raji , BCL-1 , Raw264.7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
