Application
Western Blot: 200kDa Neurofilament Heavy Antibody (NAP4) - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including (lane 2)| Product Name | NF-H antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [NAP4] to NF-HNeurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight neurofilament subunit and runs on SDS-PAGE gels in the range 180-220kDA with some variation in different species. This monoclonal recognizes specifically the phosphorylated form of NF-H which is normally found only in axons in the adult nervous system. Since closely related phosphorylated epitopes are also found on NF-M, this antibody shows some reactivity with phosphorylated forms of this protein also. Interestingly, in many damage and disease states the phosphorylated form of NF-H accumulate in the perikarya of neurons. The antibody can be used to identify axons in tissue sections and in tissue culture. It can also be used to study phosphorylated neurofilament accumulations seen in many neurological diseases and following neuronal injury. |
| Immunogen | Native pig spinal cord neurofilaments |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | NAP4 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-FIHC-PWB |
| IHC-F:1:50~1:100 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMammalian species |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | NF-H |
|---|---|
| Tissue Specificity | Specifically recognizes the phosphorylated variant of NF-H subunit (~180-220 kDa), showing some weaker reactivity with phosphorylated forms of NF-M. |
| Light Chain Type | kappa |
Application
Western Blot: 200kDa Neurofilament Heavy Antibody (NAP4) - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including (lane 2)| Application Notes | IHC-F:1:50~1:100 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | 0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA |
