Application
Immunohistochemistry staining of human cerebellum (paraffin-embedded sections) with anti-Neurofilament heavy protein (NF-01) .| Product Name | NF-H antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [NF - 01] to 200 kD Neurofilament Heavy - Neuronal MarkerNeurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight neurofilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa with some variation in different species. This monoclonal recognizes specifically the phosphorylated form of NF-H, which is normally found only in axons in the adult nervous system. Since closely related phosphorylated epitopes are also found on NF-M, this antibody shows some reactivity with phosphorylated forms of this protein also. Interestingly, in many damage and disease states the phosphorylated form of NF-H accumulate in the perikarya of neurons. The antibody can be used to identify axons in tissue sections and in tissue culture. It can also be used to study phosphorylated neurofilament accumulations seen in many neurological diseases and following neuronal injury. |
| Immunogen | A pellet of pig brain cold-stable proteins after depolymerization of microtubules. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | NF-01 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHC-FIHC-PWB |
| ELISA: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IHC-P: Use at an assay dependant dilution. IHC-Fr: Use at an assay dependant dilution. WB: Use at a dilution of 1/1000. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMousePigRatMammalian speciesBovine |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Target | 200kDa Neurofilament heavy |
|---|---|
| Tissue Specificity | This antibody recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa) of various species. |
Application
Immunohistochemistry staining of human cerebellum (paraffin-embedded sections) with anti-Neurofilament heavy protein (NF-01) .| Application Notes | ELISA: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IHC-P: Use at an assay dependant dilution. IHC-Fr: Use at an assay dependant dilution. WB: Use at a dilution of 1/1000. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
