Application
Sample (30 µg of whole cell lysate) A: 293T 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | NFIX antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NFIX (nuclear factor I/X (CCAAT-binding transcription factor))Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NFIX. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Xenopus laevisMouse (99%), Xenopus laevis (85%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NF1A antibody NFIX antibody CCAAT-box-binding transcription factor antibody nuclear factor 1 X-type antibody CTF antibody TGGCA-binding protein antibody NF1-X antibody NF-I/X antibody NFI-X antibody nuclear factor 1/X antibody nuclear factor I/X |
|---|---|
| Molecular Weight(MW) | 55 kDa (note) (4) |
Application
Sample (30 µg of whole cell lysate) A: 293T 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
